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Biomass Management

Biomass Management using Viable Cell Density Technology

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The productivity of a biological process is primarily defined by the amount of biomass generated during fermentation. Biomass measurements coupled with growth rate are critical for maintaining a steady state during a continuous process. Commonly, optical density (OD @600 nm) is used for biomass measurement which depends on the uniform scattering of light for the prediction of the number of cells in a suspension. However, the uniform light scattering is not possible if cells occur in clumps or chains such as in case of fungi, where the cells morphology is filamentous in its nature.

For more reliable real-time online measurements, varioua study was designed to evaluate Aber capacitance technology for measuring biomass. Capacitance (pF/cm) measured online was therefore compared with the offline measured. 

Filamentous Fungi https://aberinstruments.com/application/biomass-fermentation-filamentous-fungi/

Microbial https://aberinstruments.com/application/bacterial/

Challenging Microbial https://aberinstruments.com/application/real-time-viable-biomass-measurements-in-challenging-microbial-applications-using-capacitance-technology-webinar/

Filamentous Fungi

The productivity of a biological process is primarily defined by the amount of biomass generated during fermentation. Biomass measurements coupled with growth rate are critical for maintaining a steady state during a continuous process. Commonly, optical density (OD @600 nm) is used for biomass measurement which depends on the uniform scattering of light for the prediction of the number of cells in a suspension. However, the uniform light scattering is not possible if cells occur in clumps or chains such as in case of fungi, where the cells morphology is filamentous in its nature.

As such, physical methods have been employed to determine the fungal biomass, which relies on sample harvesting and off line dry cell weight measurements. This has various limitations which can affect biomass measurement/calculation and can take between 45 min to 4 hours depending on the equipment used. The physical method also fails to discriminate between biomass and necromass. Therefore, there is a requirement for the accurate measurement and control of biomass within fermenter, at both laboratory and industrial scale.

https://aberinstruments.com/application/biomass-fermentation-filamentous-fungi/

Microbial

 

One study used Capacitance via dielectric spectroscopy to monitor viable xanthamonous bacterial cells within a 2000L seed fermentor. Kelco UK was a company using 2000L fermenters in order to create Xanthamonous bacterial seeds to be transferred to a production vessel. Aber Futura systems were initially used in order to first better understand the growth of the seeds, then it was later used to help better understand when to extract the seeds.